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小鼠基质金属蛋白酶原-9(Pro-MMP-9)微量上样酶联免疫吸附检测试剂盒

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ELK8500MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	Pro-MMP-9
Assay Type:	Sandwich
Sensitivity:	0.478 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
50.00	2.225	2.143
25.00	1.590	1.508
12.50	1.029	0.947
6.25	0.850	0.768
3.13	0.486	0.404
1.56	0.363	0.281
0.78	0.173	0.091
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Pro-MMP-9 and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-MMP-9 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	98%
EDTA plasma(n=5)	91-112%	102%
Heparin plasma(n=5)	93-118%	106%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-MMP-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.