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人毒蕈碱型乙酰胆碱受体(M-AChR)酶联免疫吸附检测试剂盒

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ELK8532

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human M-AChR(Muscarinic Acetylcholine Receptor) ELISA Kit
Reactivity:	Human
Alternative Names:	M-AChR
Assay Type:	Sandwich
Sensitivity:	0.139 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Signal transduction;Infection immunity;Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M-AChR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M-AChR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M-AChR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M-AChR in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.164	2.070
10.00	1.579	1.485
5.00	1.116	1.022
2.50	0.796	0.702
1.25	0.424	0.330
0.63	0.329	0.235
0.32	0.216	0.122
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant M-AChR and the recovery rates were calculated by comparing the measured value to the expected amount of M-AChR in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-97%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of M-AChR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.