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人肺表面活性蛋白D(SP-D)微量上样酶联免疫吸附检测试剂盒

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ELK8561MS

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview

Product name:	Human SP-D(Pulmonary surfactant-associated protein D) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	SFTPD; SPD; SFTP 4;
Assay Type:	Sandwich
Sensitivity:	0.51 ng/mL
Standard:	80 ng/mL
Detection Range:	1.25-80 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Pulmonology;
Uniprot ID:	P35247
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
80.00	2.206	2.100
40.00	1.717	1.611
20.00	1.069	0.963
10.00	0.907	0.801
5.00	0.588	0.482
2.50	0.324	0.218
1.25	0.227	0.121
0.00	0.106	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SP-D and the recovery rates were calculated by comparing the measured value to the expected amount of SP-D in samples.

Matrix	Recovery range	Average
serum(n=5)	81-104%	93%
EDTA plasma(n=5)	90-107%	99%
Heparin plasma(n=5)	94-119%	107%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.