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人凝血酶原前体蛋白(PIVKA-II)微量上样酶联免疫吸附检测试剂盒

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ELK8570MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	PIVKA-II; PIVKA II
Assay Type:	Sandwich
Sensitivity:	0.13 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Hematology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.198	2.116
10.00	1.675	1.593
5.00	1.270	1.188
2.50	0.980	0.898
1.25	0.538	0.456
0.63	0.435	0.353
0.31	0.172	0.090
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PIVKA-II and the recovery rates were calculated by comparing the measured value to the expected amount of PIVKA-II in samples.

Matrix	Recovery range	Average
serum(n=5)	87-111%	99%
EDTA plasma(n=5)	81-106%	94%
Heparin plasma(n=5)	82-88%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIVKA-II and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.