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人细胞角蛋白18(CK-18)微量上样酶联免疫吸附检测试剂盒

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ELK8576MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human CK-18(Cytokeratin 18) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	Cell proliferation inducing gene 46 protein ; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein ; CK 18 ; CK-18 ; CK18 ; CYK 18 ; CYK18 ; Cytokeratin 18 ; Cytokeratin endo B ; Cytokeratin-18 ; K 18 ; K18 ; K1C18_HUMAN ; KA18 ; Keratin 18 ; Keratin 18, type I ; Keratin D ; keratin, type I cytoskeletal 18 ; Keratin-18 ; Krt18
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Cancer of the liver; Viral hepatitis
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	1.895	1.800
5.00	1.668	1.573
2.50	1.031	0.936
1.25	0.815	0.720
0.63	0.509	0.414
0.31	0.322	0.227
0.16	0.191	0.096
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CK-18 and the recovery rates were calculated by comparing the measured value to the expected amount of CK-18 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-101%	96%
EDTA plasma(n=5)	82-104%	93%
Heparin plasma(n=5)	94-115%	105%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.