Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.189 | 2.105 |
5.00 | 1.672 | 1.588 |
2.50 | 0.979 | 0.895 |
1.25 | 0.857 | 0.773 |
0.63 | 0.609 | 0.525 |
0.31 | 0.388 | 0.304 |
0.16 | 0.220 | 0.136 |
0.00 | 0.084 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-97% | 94% |
EDTA plasma(n=5) | 93-125% | 109% |
Heparin plasma(n=5) | 82-97% | 90% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 91-100% | 89-119% | 95-115% | 81-107% |
EDTA plasma(n=5) | 84-114% | 85-100% | 88-101% | 95-104% |
Heparin plasma(n=5) | 95-118% | 89-114% | 87-98% | 84-113% |
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