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小鼠FK506结合蛋白12雷帕霉素关联蛋白(FRAP)微量上样酶联免疫吸附检测试剂盒

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ELK8589MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Mouse FRAP(FK506 Binding Protein 12 Rapamycin Associated Protein) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	FRAP1; MTOR; FRAP2; MTOR; RAFT1; RAPT1; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target 1
Assay Type:	Sandwich
Sensitivity:	0.063 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;
Uniprot ID:	Q9JLN9
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FRAP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.107	2.018
5.00	1.730	1.641
2.50	1.032	0.943
1.25	0.876	0.787
0.63	0.541	0.452
0.31	0.379	0.290
0.16	0.100	0.011
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FRAP and the recovery rates were calculated by comparing the measured value to the expected amount of FRAP in samples.

Matrix	Recovery range	Average
serum(n=5)	81-113%	97%
EDTA plasma(n=5)	89-126%	108%
Heparin plasma(n=5)	94-99%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FRAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.