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人癌抗原27-29 (CA 27-29)微量上样酶联免疫吸附检测试剂盒

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ELK8602MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human CA 27-29(cancer antigen 27-29) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CA 27-29
Assay Type:	Sandwich
Sensitivity:	0.8 U/mL
Standard:	100 U/mL
Detection Range:	1.57-100 U/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cancer
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (U/mL)	OD	Corrected OD
100.00	2.110	2.033
50.00	1.512	1.435
25.00	1.212	1.135
12.50	0.922	0.845
6.25	0.571	0.494
3.13	0.267	0.190
1.56	0.212	0.135
0.00	0.077	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CA 27-29 and the recovery rates were calculated by comparing the measured value to the expected amount of CA 27-29 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-119%	104%
EDTA plasma(n=5)	86-123%	105%
Heparin plasma(n=5)	93-104%	99%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA 27-29 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.