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人白细胞抗原DR(HLADR)微量上样酶联免疫吸附检测试剂盒

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ELK8747MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human HLA-DR(Leukocyte Antigen DR) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	HALDR; Human leukocyte antigen DR
Assay Type:	Sandwich
Sensitivity:	12.5 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Immune molecule
Uniprot ID:	P01903
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-DR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-DR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-DR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-DR in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.105	2.014
1000.00	1.653	1.562
500.00	1.186	1.095
250.00	0.784	0.693
125.00	0.551	0.460
62.50	0.347	0.256
31.25	0.224	0.133
0.00	0.091	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HLA-DR and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-DR in samples.

Matrix	Recovery range	Average
serum(n=5)	81-107%	94%
EDTA plasma(n=5)	80-103%	92%
Heparin plasma(n=5)	93-100%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-DR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.