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人高敏型心肌肌钙蛋白T(hs-cTnT)微量上样酶联免疫吸附检测试剂盒

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ELK8843MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	High Sensitivity Cardiac Troponin T
Assay Type:	Sandwich
Sensitivity:	4.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.036	1.956
500.00	1.521	1.441
250.00	1.009	0.929
125.00	0.879	0.799
62.50	0.556	0.476
31.25	0.350	0.270
15.63	0.130	0.050
0.00	0.080	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.

Matrix	Recovery range	Average
serum(n=5)	86-97%	92%
EDTA plasma(n=5)	82-108%	95%
Heparin plasma(n=5)	93-109%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.