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人高敏淀粉样蛋白β1-42(Ab1-42)微量上样酶联免疫吸附检测试剂盒

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ELK8920MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	Amyloid Beta Peptide 1-42;
Assay Type:	Sandwich
Sensitivity:	1.3 pg/mL
Standard:	300 pg/mL
Detection Range:	4.69-300 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
300.00	2.152	2.067
150.00	1.658	1.573
75.00	1.169	1.084
37.50	0.896	0.811
18.75	0.542	0.457
9.38	0.384	0.299
4.69	0.171	0.086
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of hs Ab1-42 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-112%	98%
EDTA plasma(n=5)	93-120%	107%
Heparin plasma(n=5)	80-119%	100%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.