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人高敏淀粉样蛋白β1-40(Ab1-40)微量上样酶联免疫吸附检测试剂盒

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ELK8921MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human hs Ab1-40(high sensitivity Amyloid Beta Peptide 1-40) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	Amyloid Beta Peptide 1-40;
Assay Type:	Sandwich
Sensitivity:	1.42 pg/mL
Standard:	300 pg/mL
Detection Range:	4.69-300 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
300.00	2.427	2.355
150.00	1.599	1.527
75.00	1.114	1.042
37.50	0.898	0.826
18.75	0.570	0.498
9.38	0.321	0.249
4.69	0.194	0.122
0.00	0.072	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of hs Ab1-40 in samples.

Matrix	Recovery range	Average
serum(n=5)	94-117%	106%
EDTA plasma(n=5)	80-96%	88%
Heparin plasma(n=5)	88-114%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.