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小鼠新型脓毒症标志物(Presepsin)微量上样酶联免疫吸附检测试剂盒

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ELK9052MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Mouse Presepsin(Presepsin) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	soluble CD14 subtype; sCD14-ST; Presepsin
Assay Type:	Sandwich
Sensitivity:	4.72 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Sepsis
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Presepsin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Presepsin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Presepsin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Presepsin in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.260	2.179
500.00	1.411	1.330
250.00	1.108	1.027
125.00	0.910	0.829
62.50	0.562	0.481
31.25	0.365	0.284
15.63	0.257	0.176
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Presepsin and the recovery rates were calculated by comparing the measured value to the expected amount of Presepsin in samples.

Matrix	Recovery range	Average
serum(n=5)	82-115%	99%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	94-117%	106%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Presepsin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.