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人高敏铁调素25(Hepc25)微量上样酶联免疫吸附检测试剂盒

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ELK9112MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human hs-Hepc25(high sensitivity Hepcidin 25) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;
Assay Type:	Sandwich
Sensitivity:	0.61 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular; Signal Transduction; Cardiovascular; Metabolism
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	1.821	1.725
50.00	1.438	1.342
25.00	1.065	0.969
12.50	0.900	0.804
6.25	0.461	0.365
3.13	0.376	0.280
1.56	0.218	0.122
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-Hepc25 and the recovery rates were calculated by comparing the measured value to the expected amount of hs-Hepc25 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-118%	104%
EDTA plasma(n=5)	88-105%	97%
Heparin plasma(n=5)	93-99%	96%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-Hepc25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.