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人二氢嘧啶酶样蛋白2(DPYSL2)酶联免疫吸附检测试剂盒

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ELK9124

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	Human DPYSL2(Dihydropyrimidinase Like Protein 2) ELISA Kit
Reactivity:	Human
Alternative Names:	CRMP2; DHPRP2; DRP-2; DRP2; ULIP2; Unc-33-like phosphoprotein 2; Collapsin response mediator protein 2
Assay Type:	Sandwich
Sensitivity:	0.29 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample type:	tissue homogenates, cell lysates and other biological fluids
Assay length:	3.5h
Research Area:	Neuro science;
Uniprot ID:	Q16555
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPYSL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPYSL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPYSL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPYSL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Orthopaedic Surgery and Research

iTRAQ proteomic analysis of exosomes derived from synovial fluid reveals disease patterns and potential biomarkers of Osteoarthritis

日期：2025-01-17 IF：2.8

标准曲线

Concentration (ng/mL)	OD	Corrected OD
50.00	2.126	2.023
25.00	1.472	1.369
12.50	1.075	0.972
6.25	0.892	0.789
3.13	0.539	0.436
1.57	0.318	0.215
0.79	0.291	0.188
0.00	0.103	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DPYSL2 and the recovery rates were calculated by comparing the measured value to the expected amount of DPYSL2 in samples.

Matrix	Recovery range	Average
serum(n=5)	97-107%	102%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-96%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DPYSL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.