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人可溶性髓细胞触发受体1(sTREM-1)微量上样酶联免疫吸附检测试剂盒

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ELK9134MS

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview

Product name:	Human sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1
Assay Type:	Sandwich
Sensitivity:	12.4 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.3-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Immunology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.133	2.028
1000.00	1.590	1.485
500.00	1.205	1.100
250.00	0.941	0.836
125.00	0.498	0.393
62.50	0.352	0.247
31.25	0.214	0.109
0.00	0.105	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sTREM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of sTREM-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-112%	97%
EDTA plasma(n=5)	85-116%	101%
Heparin plasma(n=5)	90-115%	103%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTREM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.