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小鼠核因子κB亚基p65亲和肽(NFκB p65)酶联免疫吸附检测试剂盒

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ELK9154

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(3)

Product name:	Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Reactivity:	Mouse
Alternative Names:	NF kappa B p65delta3; NFKB3
Assay Type:	Sandwich
Sensitivity:	12.2 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample type:	serum, plasma, tissue homogenates
Assay length:	3.5h
Research Area:	Immunology
Uniprot ID:	Q04207
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

LIFE SCIENCES

Berberine attenuates epithelial mesenchymal transition in bleomycin-induced pulmonary fibrosis in mice via activating A2aR and mitigating the SDF-1/CXCR4 signaling

日期：2023-06-01 IF：6.78
Cancers

Cancer-Preventive Activity of Argemone mexicana Linn Leaves and Its Effect on TNF-α and NF-κB Signalling

日期：2023-11-21 IF：5.2
Cancers

Cancer-Preventive Activity of Argemone mexicana Linn Leaves and Interpretation of Its Orchestral Effect on TNF-α and NF-kB Signaling Modulation in DMBA/TPA Induced Skin Cancer Mice Model

日期：2023-11-21 IF：5.2

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.054	1.959
1000.00	1.527	1.432
500.00	1.251	1.156
250.00	0.834	0.739
125.00	0.453	0.358
62.50	0.326	0.231
31.25	0.187	0.092
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NFκB-p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NFκB-p65 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-107%	98%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	85-97%	91%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFκB-p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.