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人腺瘤性结肠息肉病蛋白(APC)微量上样酶联免疫吸附检测试剂盒

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ELK9156MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human APC(Adenomatous polyposis coli) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	APC; DP2; DP3; FPC; GS; DP2.5; Protein APC; Min; adenomatosis polyposis coli; adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli; adenomatous polyposis coli protein; BTPS2; Deleted in polyposis 2.5
Assay Type:	Sandwich
Sensitivity:	5.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Cell Biology; Neuroscience; Epigenetics and Nuclear Signaling
Uniprot ID:	P25054
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APC in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.119	2.030
500.00	1.632	1.543
250.00	1.209	1.120
125.00	0.959	0.870
62.50	0.574	0.485
31.25	0.375	0.286
15.63	0.172	0.083
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant APC and the recovery rates were calculated by comparing the measured value to the expected amount of APC in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	92-108%	100%
Heparin plasma(n=5)	91-104%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.