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大鼠高敏型心肌肌钙蛋白I(hs-cTnI)微量上样酶联免疫吸附检测试剂盒

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ELK9212MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Rat hs-cTnI(High Sensitivity Cardiac Troponin I) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	High Sensitivity Cardiac Troponin I
Assay Type:	Sandwich
Sensitivity:	5.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.314	2.211
500.00	1.690	1.587
250.00	1.252	1.149
125.00	0.957	0.854
62.50	0.522	0.419
31.25	0.361	0.258
15.63	0.236	0.133
0.00	0.103	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.

Matrix	Recovery range	Average
serum(n=5)	86-101%	94%
EDTA plasma(n=5)	85-114%	100%
Heparin plasma(n=5)	91-120%	106%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.