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人磷酸化神经丝蛋白H(pNF-H)酶联免疫吸附检测试剂盒

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ELK9302

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Reactivity:	Human
Alternative Names:	TC2; Transcobalamin II
Assay Type:	Sandwich
Sensitivity:	29 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay length:	3.5h
Research Area:	Neuro science
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.096	2.001
2500.00	1.587	1.492
1250.00	1.135	1.040
625.00	0.864	0.769
312.50	0.513	0.418
156.25	0.361	0.266
78.13	0.203	0.108
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant pNF-H and the recovery rates were calculated by comparing the measured value to the expected amount of pNF-H in samples.

Matrix	Recovery range	Average
serum(n=5)	82-97%	89%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	95-107%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pNF-H and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.