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人CD177分子(CD177)微量上样酶联免疫吸附检测试剂盒

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ELK9330MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human CD177(CD177 antigen) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177
Assay Type:	Sandwich
Sensitivity:	27 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	Q8N6Q3
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD177. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD177. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD177, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD177 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.077	1.977
2500.00	1.575	1.475
1250.00	1.205	1.105
625.00	0.878	0.778
312.50	0.537	0.437
156.25	0.264	0.164
78.13	0.249	0.149
0.00	0.100	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CD177 and the recovery rates were calculated by comparing the measured value to the expected amount of CD177 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-117%	100%
EDTA plasma(n=5)	84-104%	94%
Heparin plasma(n=5)	86-98%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD177 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.