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人可溶性人白细胞抗原G(sHLA-G)微量上样酶联免疫吸附检测试剂盒

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ELK9364MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human sHLA-G(soluble human leucocyte antigen G) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	sHLA-G
Assay Type:	Sandwich
Sensitivity:	0.066 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Apoptosis;Tumor immunity;Infection immunity;Immune molecule;Hematology;Reproductive science;Immunodeficiency;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sHLA-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sHLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sHLA-G in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	1.954	1.871
5.00	1.561	1.478
2.50	1.081	0.998
1.25	0.830	0.747
0.63	0.584	0.501
0.31	0.334	0.251
0.16	0.233	0.150
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sHLA-G and the recovery rates were calculated by comparing the measured value to the expected amount of sHLA-G in samples.

Matrix	Recovery range	Average
serum(n=5)	81-100%	91%
EDTA plasma(n=5)	86-96%	91%
Heparin plasma(n=5)	88-109%	99%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sHLA-G and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.