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25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒

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ELK9387

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview

Product name:	25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Reactivity:	General
Alternative Names:	25 Hydroxy Vitamin D;
Assay Type:	Competitive Inhibition
Sensitivity:	0.9 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
200.00	0.199
100.00	0.357
50.00	0.587
25.00	0.823
12.50	1.253
6.25	1.596
3.13	1.871
0.00	2.233

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	95-107%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.