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人高亲和力低亲和力神经生长因子受体(TRKA)微量上样酶联免疫吸附检测试剂盒

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ELK9487MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human TrkA/NTRK1(High affinity nerve growth factor receptor) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	NTRK1; MTC; TRK; TRKA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A;
Assay Type:	Sandwich
Sensitivity:	32 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Cell Biology; Neuroscience; Signal Transduction; Developmental Biology
Uniprot ID:	P04629
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.940	1.849
2500.00	1.578	1.487
1250.00	1.195	1.104
625.00	0.835	0.744
312.50	0.542	0.451
156.25	0.315	0.224
78.13	0.237	0.146
0.00	0.091	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TrkA/NTRK1 and the recovery rates were calculated by comparing the measured value to the expected amount of TrkA/NTRK1 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-108%	99%
EDTA plasma(n=5)	82-100%	91%
Heparin plasma(n=5)	95-103%	99%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TrkA/NTRK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.