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人前心钠肽(Pro-ANP)微量上样酶联免疫吸附检测试剂盒

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ELK9496MS

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human Pro-ANP(Pro Atrial Natriuretic Peptide) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	NT-proANP; Pro Atrial Natriuretic Peptide
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology
Uniprot ID:	P01160
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Pro-ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Pro-ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.193	2.096
5.00	1.531	1.434
2.50	1.264	1.167
1.25	0.900	0.803
0.63	0.535	0.438
0.31	0.310	0.213
0.16	0.234	0.137
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ANP in samples.

Matrix	Recovery range	Average
serum(n=5)	87-102%	95%
EDTA plasma(n=5)	86-95%	91%
Heparin plasma(n=5)	82-117%	100%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.