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游离睾酮(F-Testo)酶联免疫吸附检测试剂盒

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ELK9584

规格：	价格：
48T	￥1680.00
96T	￥2400.00

下载说明 ①

Overview

Product name:	F-Testo(Free Testosterone) ELISA Kit
Reactivity:	General
Alternative Names:	F-TESTO
Assay Type:	Competitive Inhibition
Sensitivity:	18.76 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-TESTO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-TESTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	0.198
1000.00	0.385
500.00	0.621
250.00	0.842
125.00	1.275
62.50	1.565
31.25	1.787
0.00	2.057

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant F-TESTO and the recovery rates were calculated by comparing the measured value to the expected amount of F-TESTO in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	89-103%	96%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-TESTO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.