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人酰基化饥饿素(A-GHRL)微量上样酶联免疫吸附检测试剂盒

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ELK9594MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human A-GHRL(AcylatedGhrelin) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	A-GHRL
Assay Type:	Sandwich
Sensitivity:	9.24 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular;Signal Transduction;Cardiovascular;Metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human A-GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human A-GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human A-GHRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human A-GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.993	1.909
500.00	1.531	1.447
250.00	1.248	1.164
125.00	0.953	0.869
62.50	0.506	0.422
31.25	0.350	0.266
15.63	0.189	0.105
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant A-GHRL and the recovery rates were calculated by comparing the measured value to the expected amount of A-GHRL in samples.

Matrix	Recovery range	Average
serum(n=5)	91-104%	98%
EDTA plasma(n=5)	85-96%	91%
Heparin plasma(n=5)	93-102%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A-GHRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.