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小鼠白介素27(IL-27)微量上样酶联免疫吸附检测试剂盒

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ELK9612MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Mouse IL-27(Interleukin 27) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	IL-27; IL-27A; IL27p28; IL30; MGC71873; p28;
Assay Type:	Sandwich
Sensitivity:	19.12 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Immunology;Cancer
Uniprot ID:	Q8K3I6
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.090	2.008
1000.00	1.691	1.609
500.00	1.205	1.123
250.00	0.877	0.795
125.00	0.552	0.470
62.50	0.317	0.235
31.25	0.231	0.149
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-100%	92%
EDTA plasma(n=5)	87-111%	99%
Heparin plasma(n=5)	86-115%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.