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小鼠糖抗原19-9(CA19-9)微量上样酶联免疫吸附检测试剂盒

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ELK9717MS

规格：	价格：
48T	￥1680.00
96T	￥2400.00

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Overview

Product name:	Mouse CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	sialylated Lewis(a)Antigen; Cancer Antigen 19-9
Assay Type:	Sandwich
Sensitivity:	0.27 U/mL
Standard:	50 U/mL
Detection Range:	0.79-50 U/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Tumor immunity;Hepatology;Gastroenterology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (U/mL)	OD	Corrected OD
50.00	2.150	2.056
25.00	1.568	1.474
12.50	1.210	1.116
6.25	0.868	0.774
3.13	0.585	0.491
1.56	0.320	0.226
0.78	0.235	0.141
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-111%	101%
EDTA plasma(n=5)	87-93%	90%
Heparin plasma(n=5)	91-109%	100%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.