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HSP27 (phospho Ser78) rabbit pAb

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ES1331

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

Product name:	HSP27 (phospho Ser78) rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser78. AA range:45-94
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	27kD
GeneID:	3315
Human Swiss-Prot No:	P04792
Cellular localization:	Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. .
Background:	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008],

Western Blot analysis of various cells using Phospho-HSP27 (S78) Polyclonal Antibody
Western blot analysis of lysates from HeLa cells treated with Ca2+, using HSP27 (Phospho-Ser78) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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